Double-stranded ribonucleic acid in viruses of Penicillium citrinum.

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Double-stranded ribonucleic acid in viruses of Penicillium citrinum.

The nucleic acid content of two viral populations in a strain of Penicillium citrinum is shown to be double-stranded ribonucleic acid, resolved through polyacrylamide gel electrophoresis into 10 size groups ranging from 1.17 to 3.98 megadaltons.

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The LD 50 in mice of intact virus-like particles (VLP) from Penicillium stololllferum NRRL 5267 was greater than 240 mg/kg body weight, and their component double-stranded ribonucleic acid (dsRNA) was 34·0 mg/kg. Judged by decreases in LD50 values, administration of sublethal amounts of actinomycin D or cycloheximide simultaneously with sublethal amounts of intact VLP or mycoviral dsRNA enhance...

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Characterization of Double-Stranded Ribonucleic Acid Sequences

At low ionic strength and with a low exogenous RNA polymerase/DNA ratio, rat liver chromatin directs the synthesis in vitro of RNA sequences rich in double-stranded segments. All the transcripts contain at least one double-stranded sequence. Most of the double-stranded segments are formed by intramolecular base-pairing of inverted complementary sequences separated by a single-stranded loop. The...

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Characterization of double-stranded ribonucleic acid in tobacco leaves.

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Killer strains of Saccharomyces cerevisiae contain two species of double-stranded ribonucleic acid (dsRNA) with molecular weights estimated at 2.5 x 10(6) (L) and 1.4 x 10(6) (M). The M component appears to have a high adenine content. All mutants of killer which are defective for both the toxin and immunity functions lack the M dsRNA. One of these mutants has a novel dsRNA with a molecular wei...

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ژورنال

عنوان ژورنال: Applied and Environmental Microbiology

سال: 1977

ISSN: 0099-2240,1098-5336

DOI: 10.1128/aem.34.6.811-814.1977